Cresol Red

Cresol Red
Identifiers
1733-12-6 YesY
3D model (Jmol) Interactive image
Interactive image
ChEBI CHEBI:86218 N
ChemSpider 65818 YesY
ECHA InfoCard 100.015.513
PubChem 73013
Properties
C21H17NaO5S
Molar mass 382.43 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Cresol red (full name: o-cresolsulfonephthalein) is a triarylmethane dye frequently used for monitoring the pH in aquaria.

Cresol red (pH indicator)
below pH 7.2 above pH 8.8
7.2 8.8

Molecular biology

Cresol red can be used in many common molecular biology reactions in place of other loading dyes. Cresol Red does not inhibit Taq polymerase to the same degree as other common loading dyes.

Color marker

Cresol red can also be used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. In a 1% agarose gel, it runs approximately at the size of a 125 base pair (bp) DNA molecule (it depends on the concentration of buffer and other component). Bromophenol blue and xylene cyanol can also be used for this purpose.

References

    This article is issued from Wikipedia - version of the 10/15/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.